HDAC2 Polyclonal Antibody (PA1-861) (2024)

FAQs

What are the side effects of polyclonal antibodies? ›

What is a Polyclonal Antibody? A Polyclonal Antibody represents a collection of antibodies from different B cells that recognize multiple epitopes on the same antigen. Each of these individual antibodies recognizes a unique epitope that is located on that antigen.

In contrast to polyclonal antibodies, which are produced by multiple immune cells, monoclonal antibodies are generated by identical immune cells which are clones of a single parent cell. This means that the antibody recognizes only a single epitope of an antigen and is extremely specific.

Polyclonal antibodies are produced by immunizing an animal with an antigen, collecting the serum containing the antibodies, and then purifying the antibodies. Monoclonal antibodies are produced by fusing myeloma cells and B-cells to create hybridoma cells that produce identical antibodies.

Although the polyclonal response confers advantages on the immune system, in particular, greater probability of reacting against pathogens, it also increases chances of developing certain autoimmune diseases resulting from the reaction of the immune system against native molecules produced within the host.

Polyclonal antibodies are most often used in research and testing. You might receive polyclonal antibody treatments if you're pregnant and have Rh-negative blood, get a severe infectious disease or have chronic immune thrombocytopenia.

This happens when an infection or autoimmune disease tells your immune system to keep making antibodies. Polyclonal gammopathy can develop if you have cancer or autoimmune conditions. The condition can also happen in people with liver disease.

Hypergammaglobulinemia (polyclonal gammopathy) is generally considered a benign condition that does not progress to overt malignancy, contrary to monoclonal gammopathy of undetermined significance (MGUS).

Normal immunoglobulins are called "polyclonal protein." Myeloma cells, which are cancerous plasma cells, secrete monoclonal protein. Monoclonal protein is an abnormal immunoglobulin that cannot properly fight infection. Healthy plasma cells or myeloma cells can produce immunoglobulins.

Medical Definition

polyclonal. adjective. poly·​clo·​nal ˈpäl-i-ˌklōn-ᵊl. : produced by or being cells derived from two or more cells of different ancestry or genetic constitution.

What are the applications of polyclonal antibodies? ›

Polyclonal antibodies are vital in immunology, diagnostics, and therapy because of their heterogeneous composition and versatile applications. Their ability to target multiple epitopes and enhance the immune response makes them valuable assets in the fight against disease.

Disadvantages of using Polyclonal antibodies:

Increased chance for cross reaction – false positives. Non specific interaction with the antigen- considerable heterogeneity within the antibody pool.

2.4 Polyclonal activation. Polyclonal activation is a nonspecific immune reaction. It is often observed with a high concentration of antigen that activates polyclonal B cells by binding to the LPS receptor on the surface of B cells, producing low affinity IgM-like antibodies. LPS is a polyclonal activator.

What are examples of polyclonal antibodies? Some examples of polyclonal antibodies include certain cancer treatments that can target various tumor cells. Like immunoassays such as ELISA which are frequently used in research, and immunotherapies among others.

Advantages of polyclonal antibodies

PAbs are a heterogeneous mix of antibodies that can bind to wide range of antigenic epitopes. Hence, small change in the epitopes of an antigen is less likely to affect pAbs. These antibodies are very stable across a wide range of salt concentrations and pH values.

Disadvantages of using Polyclonal antibodies:

Increased chance for cross reaction – false positives. Non specific interaction with the antigen- considerable heterogeneity within the antibody pool. Life span of the host animal is limited.

Hypergammaglobulinemia (polyclonal gammopathy) is generally considered a benign condition that does not progress to overt malignancy, contrary to monoclonal gammopathy of undetermined significance (MGUS).

These occur when your body has a strong immune response to the monoclonal antibody treatment. Common signs of infusion reaction are rash, fever, rigors/chills, shortness of breath, sweating, changes in blood pressure and increased heart rate.